Composite
Kai 1

Part:BBa_K2623010:Design

Designed by: Yiming Zhu   Group: iGEM18_XMU-China   (2018-09-28)


Kai


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 627
    Illegal AgeI site found at 1442
    Illegal AgeI site found at 2153
    Illegal AgeI site found at 2162
    Illegal AgeI site found at 2168
    Illegal AgeI site found at 2492
    Illegal AgeI site found at 2519
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used T4 DNA ligase to construct the circuit.


Source

This part contains the coding region of the Prochlorococcus (Synechococcus) elongatus PCC7942 KaiA, KaiB and KaiC gene.

References